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AChR alpha 1 antibody

Anti-AChR alpha 1 antibody used in Western Blot (WB). GTX56028
Anti-AChR alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX56028

Cat. No. GTX56028

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all AChR alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of AChR alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor nicotinic alpha 1 subunit , ACHRA , ACHRD , CHRNA , CMS1A , CMS1B , CMS2A , FCCMS , SCCMS

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The muscle acetylcholine receptor consiststs of 5 subunits of 4 different types: 2 alpha subunits and 1 each of the beta, gamma, and delta subunits. This gene encodes an alpha subunit that plays a role in acetlycholine binding/channel gating. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-AChR alpha 1 antibody used in Western Blot (WB). GTX56028

GTX56028 WB Image

WB analysis of HeLa (A), MCF7 (B), mouse kidney (C), rat liver (D) whole cell lysates using GTX56028 AChR alpha 1 antibody.

Anti-AChR alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX56028

GTX56028 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX56028 AChR alpha 1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Kazuya Furusawa et al. Researh square 2023; A cerebral organoid autonomically maintains the shape of the organic muscle cell actuators
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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399