GeneTex

  • Country / Location Selection

United States (US)

AChR alpha 3 antibody

Anti-AChR alpha 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105495
Anti-AChR alpha 3 antibody used in Western Blot (WB). GTX105495

Cat. No. GTX105495

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all AChR alpha 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Nicotinic Acetylcholine Receptor alpha 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949980

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor nicotinic alpha 3 subunit , LNCR2 , NACHRA3 , PAOD2

Cellular Localization

Cell junction , synapse , postsynaptic cell membrane , Cell membrane

Background

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AChR alpha 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105495

GTX105495 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Nicotinic Acetylcholine Receptor alpha 3(GTX105495) antibody at 1:500 dilution.

Anti-AChR alpha 3 antibody used in Western Blot (WB). GTX105495

GTX105495 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX105495 diluted at 1:1000

REFERENCE

Application Reference

Ren J et al. Int J Clin Exp Pathol 2019; 12 (9) : 3644-3652 The expression of the nicotinic acetylcholine receptor α3 subunit in the brains of patients with Alzheimer's disease and its effects on α- and γ-secretases and Notch signal transduction in SH-SY5Y cells.
Submit a Reference

REVIEW

There are currently no reviews for AChR alpha 3 antibody (GTX105495). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169