GeneTex
United States (US)

AChR alpha 4 antibody, Internal

GTX31078_2217_WB_18121410_654.jpg

Cat No. GTX31078

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX31078-PEP).

Specificity/Sensitivity

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NACHRA4 Antibody , NACRA4 Antibody , BFNC Antibody , EBN1 Antibody , CHRNA4 Antibody , NACHR Antibody , EBN Antibody

Background

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
GTX31078_2217_WB_18121410_654.jpg

GTX31078 WB Image

WB analysis of rat brain lysate using GTX31078 AChR alpha 4 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer