GeneTex
  • Country / Location Selection

United States (US)

AChR alpha 4 antibody, Internal

Anti-AChR alpha 4 antibody, Internal used in Western Blot (WB). GTX31078

Cat No. GTX31078

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX31078-PEP).

Product Note

This antibody is expected NOT to cross-react with the similar alpha 2 subunit.

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HVETRAHAEERLLKK, from the internal region of the protein sequence according to NP_000735.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cholinergic receptor nicotinic alpha 4 subunit , BFNC , EBN , EBN1 , NACHR , NACHRA4 , NACRA4

Cellular Localization

Cell membrane; Multi-pass membrane protein , Cell membrane

Background

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-AChR alpha 4 antibody, Internal used in Western Blot (WB). GTX31078

GTX31078 WB Image

WB analysis of rat brain lysate using GTX31078 AChR alpha 4 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for AChR alpha 4 antibody, Internal (GTX31078). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for AChR alpha 4 antibody, Internal (GTX31078). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 369