Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChR alpha 4 antibody [N1C1] (GTX113653) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using Nicotinic Acetylcholine Receptor alpha 4(GTX113653) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min