GeneTex

  • Country / Location Selection

United States (US)

AChR alpha 4 antibody [N1C1]

Anti-AChR alpha 4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113653
Anti-AChR alpha 4 antibody [N1C1] used in Western Blot (WB). GTX113653

Cat. No. GTX113653

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($399),
25 μl ($169)
See all AChR alpha 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AChR alpha 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor nicotinic alpha 4 subunit , BFNC , EBN , EBN1 , NACHR , NACHRA4 , NACRA4

Cellular Localization

Cell junction , synapse , postsynaptic cell membrane , Cell membrane

Background

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AChR alpha 4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113653

GTX113653 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using Nicotinic Acetylcholine Receptor alpha 4(GTX113653) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AChR alpha 4 antibody [N1C1] used in Western Blot (WB). GTX113653

GTX113653 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChR alpha 4 antibody [N1C1] (GTX113653) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xu Y et al. Aging (Albany NY) 2020; 12 (14) : 14205-14218 Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice.
Application : IHC-P
Reactivity : Mouse
Weir DL et al. Virology 2013; 444 (43102) : 21-30 Host cell tropism mediated by Australian bat lyssavirus envelope glycoproteins.
Application : WB
Reactivity : Human, Hamster
Submit a Reference

REVIEW

There are currently no reviews for AChR alpha 4 antibody [N1C1] (GTX113653). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169