APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG
Predict Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.77 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human AChR alpha 4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11169863
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cholinergic receptor nicotinic alpha 4 subunit , BFNC , EBN , EBN1 , NACHR , NACHRA4 , NACRA4
Cellular Localization
Cell junction , synapse , postsynaptic cell membrane , Cell membrane
Background
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106127 WB Image
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX106127 diluted at 1:1000
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REFERENCE
There are currently no references for AChR alpha 4 antibody [N1N3] (GTX106127). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AChR alpha 4 antibody [N1N3] (GTX106127). Be the first to share your experience with this product.