GeneTex
  • Country / Location Selection

United States (US)

AChR alpha 7 antibody, Internal

Anti-AChR alpha 7 antibody, Internal used in Western Blot (WB). GTX10096

Cat No. GTX10096

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μg ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

Jurkat
PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human AChR alpha 7

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cholinergic receptor nicotinic alpha 7 subunit , CHRNA7-2 , NACHRA7

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
Anti-AChR alpha 7 antibody, Internal used in Western Blot (WB). GTX10096

GTX10096 WB Image

WB analysis of Jurkat cells using GTX10096 AChR alpha 7 antibody at 1.25μg/ml.

REFERENCE
There are currently no references for AChR alpha 7 antibody, Internal (GTX10096). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for AChR alpha 7 antibody, Internal (GTX10096). Be the first to share your experience with this product.
Package List Price ($)
$ 329