GeneTex
United States (US)

AChR alpha 7 antibody

GTX64511_20180530_WB_18121411_372.jpg
GTX64511_20180530_IHC-P_1_18121411_345.jpg
GTX64511_20180530_IHC-P_18121411_733.jpg

Cat No. GTX64511

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NACHRA7 Antibody , CHRNA72 Antibody , CHRNA7 Antibody

Background

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
GTX64511_20180530_WB_18121411_372.jpg

GTX64511 WB Image

WB analysis of various samples using GTX64511 AChR alpha 7 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

GTX64511_20180530_IHC-P_1_18121411_345.jpg

GTX64511 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64511 AChR alpha 7 antibody.
Dilution : 1:100

GTX64511_20180530_IHC-P_18121411_733.jpg

GTX64511 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX64511 AChR alpha 7 antibody.
Dilution : 1:100