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AChR beta 2 antibody, Internal

Anti-AChR beta 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88583
Anti-AChR beta 2 antibody, Internal used in Western Blot (WB). GTX88583

Cat. No. GTX88583

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all AChR beta 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 2.5μg/ml
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88583-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence QPRHHCARQRLR, from the internal region of the protein sequence according to NP_000739.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor nicotinic beta 2 subunit , EFNL3 , nAChRB2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-AChR beta 2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88583

GTX88583 IHC-P Image

IHC-P analysis of human uterus using GTX88583 AChR beta 2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-AChR beta 2 antibody, Internal used in Western Blot (WB). GTX88583

GTX88583 WB Image

WB analysis of human cerebellum lysate using GTX88583 AChR beta 2 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399