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ADAM10 antibody [N2C1], Internal

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

Cat No. GTX104940

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

THP-1

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAM10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619636

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADAM metallopeptidase domain 10 , AD10 , AD18 , CD156c , CDw156 , HsT18717 , MADM , RAK , kuz

Cellular Localization

Cell membrane , Endomembrane system

Background

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104940

GTX104940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using ADAM10(GTX104940) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Sample (30 μg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX104940 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169