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ADAM10 antibody [N2C1], Internal

Orthogonal
Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940
Anti-ADAM10 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104940

Cat. No. GTX104940

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all ADAM10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

THP-1

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ADAM10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase domain 10 , AD10 , AD18 , CD156c , CDw156 , HsT18717 , MADM , RAK , kuz

Cellular Localization

Cell membrane , Endomembrane system

Background

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Sample (30 μg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX104940 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM10 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX104940

GTX104940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using ADAM10(GTX104940) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM10 antibody [N2C1], Internal used in Western Blot (WB). GTX104940

GTX104940 WB Image

Orthogonal

The data was published in the 2022 in Front Cell Dev Biol. PMID: 35309907

Anti-ADAM10 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104940

GTX104940 ICC/IF Image

Orthogonal

The data was published in the 2022 in Front Cell Dev Biol. PMID: 35309907

REFERENCE

Application Reference

Kumro J et al. Cereb Cortex 2023; 33 (12) : 7627-7641 Chronic basal forebrain activation improves spatial memory, boosts neurotrophin receptor expression, and lowers BACE1 and Aβ42 levels in the cerebral cortex in mice.
Yan Zhang et al. Front Cell Dev Biol 2022; 10 : 829404 Mechanisms of Senescence-Related NKG2D Ligands Release and Immune Escape Induced by Chemotherapy in Neuroblastoma Cells.
Application : WB, ICC/IF
Reactivity : Human
Groot AJ et al. Mol Cell Biol 2014; 34 (15) : 2822-32 Regulated proteolysis of NOTCH2 and NOTCH3 receptors by A-Disintegrin-And-Metalloproteinase (ADAM) 10 and Presenilins.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169