GeneTex
United States (US)

ADAM10 antibody [N2C1], Internal

GTX104940_40051_IHC_19011506_392.jpg
GTX104940_40051_WB_19011506_394.jpg

Cat No. GTX104940

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

THP-1

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAM10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AD10 antibody, CD156c antibody, HsT18717 antibody, MADM antibody, kuz antibody, ADAM10 antibody, mammalian disintegrin-metalloprotease antibody, a disintegrin and metalloproteinase domain 10 antibody, kuzbanian protein homolog antibody, CDw156 antibody, disintegrin and metalloproteinase domain-containing protein 10 antibody, ADAM 10 antibody, a disintegrin and metalloprotease domain 10 antibody, ADAM metallopeptidase domain 10 antibody

Cellular Localization

Cell membrane , Endomembrane system

Background

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104940_40051_IHC_19011506_392.jpg

GTX104940 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using ADAM10(GTX104940) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104940_40051_WB_19011506_394.jpg

GTX104940 WB Image

Sample (30 µg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX104940 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169