Untreated (–) and treated (+) unboiled A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled RT-4 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(WCE: whole cell extract; ME: membrane extract)

Immunohistochemical analysis of paraffin-embedded human lung Papillary adenocarcinoma, using ADAM10(GTX104940) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX104940 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM10 antibody [N2C1], Internal (GTX104940) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the 2022 in Front Cell Dev Biol. PMID: 35309907

The data was published in the 2022 in Front Cell Dev Biol. PMID: 35309907