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ADAM10 antibody

Anti-ADAM10 antibody used in Western Blot (WB). GTX21997
Anti-ADAM10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21997
Anti-ADAM10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21997

Cat No. GTX21997

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF 2-10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , Jurkat Cell Lysate(GTX27899)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 732-748 of Human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADAM metallopeptidase domain 10 , AD10 , AD18 , CD156c , CDw156 , HsT18717 , MADM , RAK , kuz

Cellular Localization

Cell membrane,Golgi apparatus membrane

Background

Proinflammatory cytokine tumor necrosis factor-a (TNF-a)contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, Respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Database

Research Area

DATA IMAGES

Anti-ADAM10 antibody used in Western Blot (WB). GTX21997

GTX21997 WB Image

WB analysis of Jurkat cell lysate using GTX21997 ADAM10 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-ADAM10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21997

GTX21997 ICC/IF Image

ICC/IF analysis of K562 cells using GTX21997 ADAM10 antibody.
Working concentration : 2 μg/ml

Anti-ADAM10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21997

GTX21997 ICC/IF Image

ICC/IF analysis of K562 cells using GTX21997 ADAM10 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399