APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 10μg/ml |
| 3.75μg/ml |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm and plasma membrane of A431 cells.
IHC-P
Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89596-PEP).
Product Note
This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465.3; NP_067673.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADAM metallopeptidase domain 12 , ADAM12-OT1 , CAR10 , MCMP , MCMPMltna , MLTN , MLTNA
Background
This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
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GTX89596 IHC-P Image
IHC-P analysis of human prostate using GTX89596 ADAM12 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX89596 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX89596 ADAM12 antibody, N-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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GTX89596 ICC/IF Image
ICC/IF analysis of PFA fixed A431 cells using GTX89596 ADAM12 antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89596 IHC-P Image
IHC-P analysis of human placenta using GTX89596 ADAM12 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
|
REFERENCE
There are currently no references for ADAM12 antibody, N-term (GTX89596). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ADAM12 antibody, N-term (GTX89596). Be the first to share your experience with this product.
