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ADAM12 antibody

Anti-ADAM12 antibody used in IHC (Paraffin sections) (IHC-P). GTX55491
Anti-ADAM12 antibody used in Western Blot (WB). GTX55491
Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491
Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491
Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491

Cat. No. GTX55491

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ADAM12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 589-738 of human ADAM12 (NP_067673.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase domain 12 , ADAM12-OT1 , CAR10 , MCMP , MCMPMltna , MLTN , MLTNA

Background

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ADAM12 antibody used in IHC (Paraffin sections) (IHC-P). GTX55491

GTX55491 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55491 ADAM12 antibody.
Dilution : 1:100

Anti-ADAM12 antibody used in Western Blot (WB). GTX55491

GTX55491 WB Image

WB analysis of various sample lysates using GTX55491 ADAM12 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491

GTX55491 ICC/IF Image

ICC/IF analysis of C6 cells using GTX55491 ADAM12 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491

GTX55491 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55491 ADAM12 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ADAM12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55491

GTX55491 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55491 ADAM12 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399