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ADAM15 antibody [N1N2], N-term

Anti-ADAM15 antibody [N1N2], N-term used in Western Blot (WB). GTX101599
Anti-ADAM15 antibody [N1N2], N-term used in Western Blot (WB). GTX101599
Anti-ADAM15 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101599

Cat No. GTX101599

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa , HepG2 , Molt-4 , NIH-3T3
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.347 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ADAM15. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADAM metallopeptidase domain 15 , MDC15

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ADAM15 antibody [N1N2], N-term used in Western Blot (WB). GTX101599

GTX101599 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
ADAM15 antibody
GTX101599 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM15 antibody [N1N2], N-term used in Western Blot (WB). GTX101599

GTX101599 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX101599 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM15 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101599

GTX101599 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using ADAM15(GTX101599) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169