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ADAM17 antibody [C2C3], C-term

KO/KD
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

Cat. No. GTX101358

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ADAM17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

K562 , Raw264.7 , Rat2 , HeLa

Predict Reactivity

Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human ADAM17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036165

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE

Cellular Localization

Membrane

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

KO/KD

Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract |
7.5% SDS-PAGE
ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101358

GTX101358 IHC-P Image

ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Yu W et al. Am J Physiol Gastrointest Liver Physiol 2023; 324 (1) : G24-G37 Short-chain fatty acids ameliorate necrotizing enterocolitis-like intestinal injury through enhancing Notch1-mediated single immunoglobulin interleukin-1-related receptor, toll-interacting protein, and A20 induction.
Khoury EE et al. J Cell Mol Med 2021; 25 (8) : 3840-3855 Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease.
Application : WB
Reactivity : Rat
Arcidiacono MV et al. Nephrol Dial Transplant 2015; 30 (3) : 423-33 The induction of C/EBPβ contributes to vitamin D inhibition of ADAM17 expression and parathyroid hyperplasia in kidney disease.
Application : WB
Reactivity : Human
Groot AJ et al. Mol Cell Biol 2014; Regulated proteolysis of NOTCH2 and NOTCH3 receptors by A-Disintegrin-And-Metalloproteinase (ADAM) 10 and Presenilins.
Application : WB
Reactivity : Human
Yoshida H et al. Neurosci Res 2013; Carnosic acid suppresses the production of amyloid-β 1-42 and 1-43 by inducing an α-secretase TACE/ADAM17 in U373MG human astrocytoma cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169