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ADAM17 antibody [C2C3], C-term

KO/KD
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101358
Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

Cat No. GTX101358

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all ADAM17 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

HeLa , K562

Predict Reactivity

Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ADAM17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036165

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE

Cellular Localization

Membrane

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

KO/KD

Wild-type (WT) and ADAM17 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

ADAM17 antibody [C2C3], C-term detects ADAM17 protein by western blot analysis.
A. 30 μg K562 whole cell lysate/extract |
7.5% SDS-PAGE
ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAM17 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101358

GTX101358 IHC-P Image

ADAM17 antibody [C2C3], C-term detects ADAM17 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: ADAM17 antibody [C2C3], C-term (GTX101358) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAM17 antibody [C2C3], C-term used in Western Blot (WB). GTX101358

GTX101358 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term (GTX101358) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Khoury EE et al. J Cell Mol Med 2021; 25 (8) : 3840-3855 Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease.
Application : WB
Reactivity : Rat
Arcidiacono MV et al. Nephrol Dial Transplant 2015; 30 (3) : 423-33 The induction of C/EBPβ contributes to vitamin D inhibition of ADAM17 expression and parathyroid hyperplasia in kidney disease.
Application : WB
Reactivity : Human
Groot AJ et al. Mol Cell Biol 2014; Regulated proteolysis of NOTCH2 and NOTCH3 receptors by A-Disintegrin-And-Metalloproteinase (ADAM) 10 and Presenilins.
Application : WB
Reactivity : Human
Yoshida H et al. Neurosci Res 2013; Carnosic acid suppresses the production of amyloid-β 1-42 and 1-43 by inducing an α-secretase TACE/ADAM17 in U373MG human astrocytoma cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169