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ADAM17 antibody [D1(A12)] (Azide free)

Anti-ADAM17 antibody [D1(A12)] (Azide free) used in Functional Assay (Functional Assay). GTX33911

Cat No. GTX33911

Host Human
Clonality Monoclonal
Clone Name D1(A12)
Isotype IgG1
Application Functional Assay
Reactivity Human
APPLICATION

Application Note

FUNC (Blocking): Inhibits ADAM17 activity at 15μg/ml (200nm). Optimal conditions should be determined individually for each application.

Calculated MW

93 kDa. ( Note )

Specificity/Sensitivity

Recognizes the catalytic and non-catalytic domain of human ADAM17 (TACE) through its variable light (VL) domain and variable heavy (VH) domain, respectively. Does not bind recombinant mouse ADAM17 ectodomain.
PROPERTIES

Form

Liquid

Buffer

PBS.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human ADAM17 (TACE) ectodomain tagged to biotin.

Purification

Protein A/G-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adam Metallopeptidase Domain 17,Adam18,Cd156B,Csvp,Nisbd,Nisbd1,Tace,Adam17

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-ADAM17 antibody [D1(A12)] (Azide free) used in Functional Assay (Functional Assay). GTX33911

GTX33911 Functional Assay Image

D1(A12) IgG inhibits constitutive shedding of TNF-α from IGROV1 (human ovarian cancer cell line) into culture medium. Medium was collected after 48 hours of incubation with or without IgGs at 200nM.

Package List Price ($)
$ 419