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ADAM17 antibody

Anti-ADAM17 antibody used in Western Blot (WB). GTX13535
Anti-ADAM17 antibody used in IHC (Paraffin sections) (IHC-P). GTX13535
Anti-ADAM17 antibody used in IHC (Paraffin sections) (IHC-P). GTX13535
Anti-ADAM17 antibody used in Western Blot (WB). GTX13535

Cat No. GTX13535

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX13535-PEP).

Product Note

This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence LQRQNRVDSKETEC, from the C Terminus of the protein sequence according to NP_003174.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-ADAM17 antibody used in Western Blot (WB). GTX13535

GTX13535 WB Image

WB analysis of Daudi (A), Jurkat (B) and HeLa (C) lysates using GTX13535 ADAM17 antibody.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-ADAM17 antibody used in IHC (Paraffin sections) (IHC-P). GTX13535

GTX13535 IHC-P Image

IHC-P analysis of human skin using GTX13535 ADAM17 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-ADAM17 antibody used in IHC (Paraffin sections) (IHC-P). GTX13535

GTX13535 IHC-P Image

IHC-P analysis of human thyroid using GTX13535 ADAM17 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-ADAM17 antibody used in Western Blot (WB). GTX13535

GTX13535 WB Image

WB analysis of human testis lysate using GTX13535 ADAM17 antibody.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for ADAM17 antibody (GTX13535). Be the first to share your publications with this product.

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319