GeneTex
United States (US)

ADAM17 antibody

GTX31632_2814_WB_20180221_18121410_456.jpg
GTX31632_228_ICC-IF_20180221_18121410_226.jpg
GTX31632_229_ICC-IF_20180221_18121410_101.jpg

Cat No. GTX31632

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ICC/IF 10 μg/mL
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

4)HeLa Cell Slide(GTX25529) , Jurkat Cell Lysate(GTX27899) , Raji Cell Lysate(GTX27908)

Specificity/Sensitivity

80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated form.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ADAM17 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human ADAM17.The immunogen is located within the last 50 amino acids of ADAM17.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adam Metallopeptidase Domain 17,Adam18,Cd156B,Csvp,Nisbd,Nisbd1,Tace,Adam17

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31632_2814_WB_20180221_18121410_456.jpg

GTX31632 WB Image

WB analysis of HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide using GTX31632 ADAM17 antibody.
Working concentration : 0.5 μg/ml

GTX31632_228_ICC-IF_20180221_18121410_226.jpg

GTX31632 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31632 ADAM17 antibody.
Working concentration : 10 μg/ml

GTX31632_229_ICC-IF_20180221_18121410_101.jpg

GTX31632 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31632 ADAM17 antibody.
Working concentration : 10 μg/ml

Package List Price ($)
$ 329