APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
4)HeLa Cell Slide(GTX25529) , Jurkat Cell Lysate(GTX27899) , Raji Cell Lysate(GTX27908)
Product Note
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated form.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ADAM17 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human ADAM17.The immunogen is located within the last 50 amino acids of ADAM17.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADAM metallopeptidase domain 17 , ADAM18 , CD156B , CSVP , NISBD , NISBD1 , TACE
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31632 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31632 ADAM17 antibody.
Working concentration : 10 μg/ml
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GTX31632 WB Image
WB analysis of HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide using GTX31632 ADAM17 antibody.
Working concentration : 0.5 μg/ml
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GTX31632 WB Image
WB analysis of various sample lysates using GTX31632 ADAM17 antibody.
Loading : 15μg of lysates per lane
Dilution : 0.5 μg/ml
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GTX31632 WB Image
WB analysis of HeLa cell or ADAM17 KO cell lysates using GTX31632 ADAM17 antibody.
Dilution : 0.25μg/mL
Loading : 10μg
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GTX31632 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31632 ADAM17 antibody.
Working concentration : 10 μg/ml
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REFERENCE
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REVIEW
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