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ADAM1B antibody [#158]

Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690
Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690
Anti-ADAM1B antibody [#158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00690

Cat No. GTX00690

Host

Rat

Clonality

Monoclonal

Clone Name

#158

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Mouse
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:500
ICC/IF 1:300
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Specificity/Sensitivity

This antibody showed no reactivity with ADAM1A (confirmed by WB using recombinant ADAM1A and ADAM1B proteins).

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse sperm

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

a disintegrin and metallopeptidase domain 1b , Adam1 , Ftna

Cellular Localization

Membrane

Database

DATA IMAGES

Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690

GTX00690 WB Image

WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence.
This protien is testis-specific.
Dilution : 1:100
This protien is testis-specific.

Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690

GTX00690 WB Image

WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158].
ADAM1B appeared as a faint signal at 3 weeks of age.

Anti-ADAM1B antibody [#158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00690

GTX00690 ICC/IF Image

ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158].
Dilution : 1:300
Fixation : 4% PFA

REFERENCE

REVIEW

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Package List Price ($)
$ 359