GeneTex
United States (US)

ADAMTS4 antibody [N3C2], Internal

GTX105125_40142_20150910_WB_M_brain_19011506_956.jpg

Cat No. GTX105125

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAMTS4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ADAMTS-2 antibody, ADAMTS-4 antibody, ADMP-1 antibody, KIAA0688 antibody, ADAMTS4 antibody, ADAM-TS4 antibody, "a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 antibody", A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody, aggrecanase-1 antibody, ADAM-TS 4 antibody, "ADAM metallopeptidase with thrombospondin type 1 motif, 4 antibody"

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq]
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Database

DATA IMAGES
GTX105125_40142_20150910_WB_M_brain_19011506_956.jpg

GTX105125 WB Image

ADAMTS4 antibody detects ADAMTS4 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS4 antibody (GTX105125) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169