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ADAMTS5 antibody [N3C2], Internal

Orthogonal
Anti-ADAMTS5 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX123657
Anti-ADAMTS5 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX123657
Anti-ADAMTS5 antibody [N3C2], Internal used in Western Blot (WB). GTX123657
Anti-ADAMTS5 antibody [N3C2], Internal used in Western Blot (WB). GTX123657

Cat. No. GTX123657

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ADAMTS5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HeLa , HepG2 , PC-12 , Rat-2

Predict Reactivity

Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAMTS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162317

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase with thrombospondin type 1 motif 5 , ADAM-TS 11 , ADAM-TS 5 , ADAM-TS5 , ADAMTS-11 , ADAMTS-5 , ADAMTS11 , ADMP-2

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAMTS5 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX123657

GTX123657 IHC-P Image

ADAMTS5 antibody [N3C2], Internal detects ADAMTS5 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
ADAMTS5 antibody [N3C2], Internal (GTX123657) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAMTS5 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX123657

GTX123657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADAMTS5(GTX123657) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAMTS5 antibody [N3C2], Internal used in Western Blot (WB). GTX123657

GTX123657 WB Image

Orthogonal

Untreated (–) and treated (+) RMS 13 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal (GTX123657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 95 kDa, and 120 kDa. It is possibly due to alternative splicing.
Reference: PMID: 18992360

Anti-ADAMTS5 antibody [N3C2], Internal used in Western Blot (WB). GTX123657

GTX123657 WB Image

Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal (GTX123657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 90 kDa, and 110 kDa. It is possibly due to alternative splicing.
Reference: PMID: 18992360

REFERENCE

Application Reference

Bai H et al. J Cell Mol Med 2022; 26 (15) : 4230-4243 Activation of adenosine A3 receptor attenuates progression of osteoarthritis through inhibiting the NLRP3/caspase-1/GSDMD induced signalling.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169