GeneTex
United States (US)

ADAMTS5 antibody [N3C2], Internal

GTX123657_40737_20170330_WB_R_18121410_751.jpg
GTX123657_40737_IHC_18121410_740.jpg
GTX123657_40737_CT_IHC_18121410_511.jpg
GTX123657_40737_WB_18121410_963.jpg

Cat No. GTX123657

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HeLa , HepG2 , PC-12 , Rat-2

Predict Reactivity

Mouse, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAMTS5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adam Metallopeptidase With Thrombospondin Type 1 Motif 5,Adam-Ts 11,Adam-Ts 5,Adam-Ts5,Adamts-11,Adamts-5,Adamts11,Admp-2,Adamts5

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq]

Database

DATA IMAGES
GTX123657_40737_20170330_WB_R_18121410_751.jpg

GTX123657 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAMTS5 antibody [N3C2], Internal (GTX123657) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX123657_40737_IHC_18121410_740.jpg

GTX123657 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADAMTS5(GTX123657) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123657_40737_CT_IHC_18121410_511.jpg

GTX123657 IHC-P Image

ADAMTS5 antibody [N3C2], Internal detects ADAMTS5 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
ADAMTS5 antibody [N3C2], Internal (GTX123657) dilution: 1:250.
Scale bar = 100 μm.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123657_40737_WB_18121410_963.jpg

GTX123657 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
7.5% SDS PAGE
GTX123657 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169