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ADAMTS9 antibody

Anti-ADAMTS9 antibody used in Western Blot (WB). GTX79416
Anti-ADAMTS9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79416

Cat. No. GTX79416

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/ml
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

216 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C F D G K H F N I N G L L P N of human ADAMTS9.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11164895

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADAM metallopeptidase with thrombospondin type 1 motif 9

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-ADAMTS9 antibody used in Western Blot (WB). GTX79416

GTX79416 WB Image

WB analysis of TS9 transfected HEK-293 cell lysate(lane 1) and untreated cell lysate(lane 2) using GTX79416 ADAMTS9 antibody.

Anti-ADAMTS9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79416

GTX79416 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX79416 ADAMTS9 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399