APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888087
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
adenosine deaminase RNA specific , ADAR1 , AGS6 , DRADA , DSH , DSRAD , G1P1 , IFI-4 , IFI4 , K88DSRBP , P136
Cellular Localization
Cytoplasm , Nucleus , nucleolus
Background
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000](/templates/default/js/lightbox/images/loading.gif "Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000")
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GTX629000 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:500.
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GTX629000 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629000 IP Image
Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [GT1066] (GTX629000).
Western blot analysis was performed using ADAR1 antibody [GT1066] (GTX629000).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX629000 ICC/IF Image
ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: ADAR1 protein stained by ADAR1 antibody [GT1066] (GTX629000) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.
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REFERENCE
There are currently no references for ADAR1 antibody [GT1066] (GTX629000). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ADAR1 antibody [GT1066] (GTX629000). Be the first to share your experience with this product.
![Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000](/upload/website/prouct_img/small/GTX629000/GTX629000_41288_20160922_WB_shRNA_watermark_w_23061202_446.webp "Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000](/upload/website/prouct_img/small/GTX629000/GTX629000_44503_20211112_WB_w_23061202_289.webp "Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Immunoprecipitation (IP). GTX629000](/upload/website/prouct_img/small/GTX629000/GTX629000_41288_20150612_IP_w_23061202_753.webp "Anti-ADAR1 antibody [GT1066] used in Immunoprecipitation (IP). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629000](/upload/website/prouct_img/small/GTX629000/GTX629000_41288_IFA_w_23061202_642.webp "Anti-ADAR1 antibody [GT1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000](/upload/website/prouct_img/normal/GTX629000/GTX629000_41288_20160922_WB_shRNA_watermark_w_23061202_446.webp "Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000](/upload/website/prouct_img/normal/GTX629000/GTX629000_44503_20211112_WB_w_23061202_289.webp "Anti-ADAR1 antibody [GT1066] used in Western Blot (WB). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Immunoprecipitation (IP). GTX629000](/upload/website/prouct_img/normal/GTX629000/GTX629000_41288_20150612_IP_w_23061202_753.webp "Anti-ADAR1 antibody [GT1066] used in Immunoprecipitation (IP). GTX629000")
![Anti-ADAR1 antibody [GT1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629000](/upload/website/prouct_img/normal/GTX629000/GTX629000_41288_IFA_w_23061202_642.webp "Anti-ADAR1 antibody [GT1066] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629000")