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ADAR1 antibody [HL1789]

Recombinant antibody KO/KD
Anti-ADAR1 antibody [HL1789] used in Western Blot (WB). GTX637441
Anti-ADAR1 antibody [HL1789] used in IHC (Paraffin sections) (IHC-P). GTX637441
Anti-ADAR1 antibody [HL1789] used in Western Blot (WB). GTX637441

Cat. No. GTX637441

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1789

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ADAR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human ADAR1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine deaminase RNA specific , ADAR1 , AGS6 , DRADA , DSH , DSRAD , G1P1 , IFI-4 , IFI4 , K88DSRBP , P136

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-ADAR1 antibody [HL1789] used in Western Blot (WB). GTX637441

GTX637441 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [HL1789] (GTX637441) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAR1 antibody [HL1789] used in IHC (Paraffin sections) (IHC-P). GTX637441

GTX637441 IHC-P Image

ADAR1 antibody [HL1789] detects ADAR1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
ADAR1 stained by ADAR1 antibody [HL1789] (GTX637441) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADAR1 antibody [HL1789] used in Western Blot (WB). GTX637441

GTX637441 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [HL1789] (GTX637441) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations