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ADAR1 antibody [N3C1], Internal

KO/KD
Anti-ADAR1 antibody [N3C1], Internal used in Western Blot (WB). GTX101602
Anti-ADAR1 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX101602
Anti-ADAR1 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101602
Anti-ADAR1 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101602
Anti-ADAR1 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101602
Anti-ADAR1 antibody [N3C1], Internal used in Western Blot (WB). GTX101602

Cat. No. GTX101602

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($419),
25 μl ($169)
See all ADAR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine deaminase RNA specific , ADAR1 , AGS6 , DRADA , DSH , DSRAD , G1P1 , IFI-4 , IFI4 , K88DSRBP , P136

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAR1 antibody [N3C1], Internal used in Western Blot (WB). GTX101602

GTX101602 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADAR1 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX101602

GTX101602 IP Image

Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [N3C1], Internal (GTX101602).
Western blot analysis was performed using ADAR1 antibody [N3C1], Internal (GTX101602).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ADAR1 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101602

GTX101602 ICC/IF Image

ADAR1 antibody [N3C1], Internal detects ADAR1 protein at nucleolus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ADAR1 protein stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ADAR1 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101602

GTX101602 IHC-P Image

ADAR1 antibody [N3C1], Internal detects ADAR1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ADAR1 stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADAR1 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX101602

GTX101602 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ADAR1(GTX101602) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADAR1 antibody [N3C1], Internal used in Western Blot (WB). GTX101602

GTX101602 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169