*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Mouse, Rat(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human ADAR1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
adenosine deaminase RNA specific , ADAR1 , AGS6 , DRADA , DSH , DSRAD , G1P1 , IFI-4 , IFI4 , K88DSRBP , P136
Cytoplasm , Nucleus , nucleolus
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
GTX101602 IP Image
Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [N3C1], Internal (GTX101602).
Western blot analysis was performed using ADAR1 antibody [N3C1], Internal (GTX101602).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX101602 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101602 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ADAR1(GTX101602) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101602 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101602 ICC/IF Image
ADAR1 antibody [N3C1], Internal detects ADAR1 protein at nucleolus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ADAR1 protein stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.