Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 μg of ADAR1 antibody [N3C1], Internal (GTX101602).
Western blot analysis was performed using ADAR1 antibody [N3C1], Internal (GTX101602).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ADAR1(GTX101602) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ADAR1 antibody [N3C1], Internal detects ADAR1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ADAR1 stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ADAR1 antibody [N3C1], Internal detects ADAR1 protein at nucleolus and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ADAR1 protein stained by ADAR1 antibody [N3C1], Internal (GTX101602) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.