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ADAR2 antibody

Anti-ADAR2 antibody used in Western Blot (WB). GTX114237
Anti-ADAR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114237

Cat. No. GTX114237

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ADAR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADAR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2616090

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

adenosine deaminase RNA specific B1 , ADAR2 , DRABA2 , DRADA2 , RED1

Background

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADAR2 antibody used in Western Blot (WB). GTX114237

GTX114237 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
GTX114237 diluted at 1:1000

Anti-ADAR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114237

GTX114237 ICC/IF Image

ADAR2 antibody detects ADAR2 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ADAR2 protein stained by ADAR2 antibody (GTX114237) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Chen J et al. EMBO Rep 2020; 21 (4) : e48467 Androgen receptor-regulated circFNTA activates KRAS signaling to promote bladder cancer invasion.
Freund EC et al. Cell Rep 2020; 31 (7) : 107656 Unbiased Identification of trans Regulators of ADAR and A-to-I RNA Editing.
Emily C. Freund et al. bioRxiv 2019; (Epub) Unbiased identification of trans regulators of ADAR and A-to-I RNA editing.
Application : WB
Reactivity : Human
Wang Y et al. Genome Res 2017; (Epub) Systematic characterization of A-to-I RNA editing hotspots in microRNAs across human cancers.
Application : WB
Reactivity : Human
Shoshan E et al. Nat Cell Biol 2015; 17 (3) : 311-21 Reduced adenosine-to-inosine miR-455-5p editing promotes melanoma growth and metastasis.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169