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ADAR2 antibody

Anti-ADAR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54916
Anti-ADAR2 antibody used in Western Blot (WB). GTX54916
Anti-ADAR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54916

Cat. No. GTX54916

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ADAR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate pH7.3, 0.87% Sodium Chloride, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 496 and 535 of human ADAR2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenosine deaminase RNA specific B1 , ADAR2 , DRABA2 , DRADA2 , RED1

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes the enzyme responsible for pre-mRNA editing of the glutamate receptor subunit B by site-specific deamination of adenosines. Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ADAR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54916

GTX54916 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX54916 ADAR2 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-ADAR2 antibody used in Western Blot (WB). GTX54916

GTX54916 WB Image

WB analysis of HepG2 (A), MCF7 (B), mouse brain (C), rat brain (D) whole cell lysates using GTX54916 ADAR2 antibody.

Anti-ADAR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX54916

GTX54916 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX54916 ADAR2 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

V Sekar et al. Nat Biotechnol 2023; Detection of transcriptome-wide microRNA–target interactions in single cells with agoTRIBE
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399