ADFP antibody [ADFP/1365]

Cat. No. GTX34395

Host	Mouse
Clonality	Monoclonal
Clone Name	ADFP/1365
Isotype	IgG1
Application	WB, FACS, ELISA, Protein Array
Reactivity	Human
Package	100 μg ($529)

See all ADFP products

Datasheet
Document

View product citations for antibody GTX34395 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
FACS	1-2μg/10⁶ cells
ELISA	Assay dependent
Protein Array	Assay dependent
Note : ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADFP , ADRP , PLIN2 , perilipin 2 , Perilipin 2 , Adipophilin

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Gene ID: 123 PLIN2
UniProt: Q99541 PLIN2

Research Area

- Cancer
- Cancer
- Hypoxia

DATA IMAGES

GTX34395 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Adipophilin Mouse Monoclonal Antibody (ADFP/1365). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

GTX34395 WB Image

WB analysis of recombinant Adipophilin protein and Jurkat cell lysates using GTX34395 ADFP antibody [ADFP/1365].

GTX34395 FACS Image

FACS analysis of PBMCs using GTX34395 ADFP antibody [ADFP/1365].
Red : Primary antibody
Green : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty