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ADFP antibody [ADFP/2755R]

Anti-ADFP antibody [ADFP/2755R] used in IHC (Paraffin sections) (IHC-P). GTX02691
Anti-ADFP antibody [ADFP/2755R] used in Western Blot (WB). GTX02691

Cat. No. GTX02691

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 ADFP/2755R

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($529)
See all ADFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IHC-P 1-2 μg/ml
ELISA Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perilipin 2 , ADFP , ADRP

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-ADFP antibody [ADFP/2755R] used in IHC (Paraffin sections) (IHC-P). GTX02691

GTX02691 IHC-P Image

IHC-P analysis of human adrenal gland section using GTX02691 ADFP antibody [ADFP/2755R].

Anti-ADFP antibody [ADFP/2755R] used in Western Blot (WB). GTX02691

GTX02691 WB Image

WB analysis of human liver tissue lysate using GTX02691 ADFP antibody [ADFP/2755R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 529