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ADFP antibody [HL2145]

Recombinant antibody
Anti-ADFP antibody [HL2145] used in Western Blot (WB). GTX638123
Anti-ADFP antibody [HL2145] used in IHC (Paraffin sections) (IHC-P). GTX638123
Anti-ADFP antibody [HL2145] used in Western Blot (WB). GTX638123

Cat. No. GTX638123

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2145

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ADFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human ADFP

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perilipin 2 , ADFP , ADRP

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-ADFP antibody [HL2145] used in Western Blot (WB). GTX638123

GTX638123 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2145] (GTX638123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ADFP antibody [HL2145] used in IHC (Paraffin sections) (IHC-P). GTX638123

GTX638123 IHC-P Image

ADFP antibody [HL2145] detects ADFP protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
ADFP stained by ADFP antibody [HL2145] (GTX638123) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ADFP antibody [HL2145] used in Western Blot (WB). GTX638123

GTX638123 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2145] (GTX638123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations