APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragmemt of human ADFP
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
perilipin 2 , ADFP , ADRP
Cellular Localization
Membrane
Background
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/templates/default/js/lightbox/images/loading.gif "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
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GTX638125 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (GTX638125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638125 ICC/IF Image
ADFP antibody [HL2147] detects ADFP protein at lipid droplet by immunofluorescent analysis.Sample: Mock and differentiated 3T3-L1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ADFP stained by ADFP antibody [HL2147] (GTX638125) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638125 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (GTX638125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638125 IHC-P Image
ADFP antibody [HL2147] detects ADFP protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.ADFP stained by ADFP antibody [HL2147] (GTX638125) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638125 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [HL2147] (GTX638125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for ADFP antibody [HL2147] (GTX638125). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ADFP antibody [HL2147] (GTX638125). Be the first to share your experience with this product.
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/small/GTX638125/GTX638125_45012_20230526_WB_R_23053001_848.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
![Anti-ADFP antibody [HL2147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638125](/upload/website/prouct_img/small/GTX638125/GTX638125_45012_20231006_ICC_IF_M_differentiation_23102401_900.webp "Anti-ADFP antibody [HL2147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638125")
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/small/GTX638125/GTX638125_T-44928_20230210_WB_M_tissue_23021401_355.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
![Anti-ADFP antibody [HL2147] used in IHC (Paraffin sections) (IHC-P). GTX638125](/upload/website/prouct_img/small/GTX638125/GTX638125_T-44928_20230217_IHC-P_multiple_M_23021401_288.webp "Anti-ADFP antibody [HL2147] used in IHC (Paraffin sections) (IHC-P). GTX638125")
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/small/GTX638125/GTX638125_45012_20230421_WB_23042500_668.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/normal/GTX638125/GTX638125_45012_20230526_WB_R_23053001_848.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
![Anti-ADFP antibody [HL2147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638125](/upload/website/prouct_img/normal/GTX638125/GTX638125_45012_20231006_ICC_IF_M_differentiation_23102401_900.webp "Anti-ADFP antibody [HL2147] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638125")
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/normal/GTX638125/GTX638125_T-44928_20230210_WB_M_tissue_23021401_355.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")
![Anti-ADFP antibody [HL2147] used in IHC (Paraffin sections) (IHC-P). GTX638125](/upload/website/prouct_img/normal/GTX638125/GTX638125_T-44928_20230217_IHC-P_multiple_M_23021401_288.webp "Anti-ADFP antibody [HL2147] used in IHC (Paraffin sections) (IHC-P). GTX638125")
![Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125](/upload/website/prouct_img/normal/GTX638125/GTX638125_45012_20230421_WB_23042500_668.webp "Anti-ADFP antibody [HL2147] used in Western Blot (WB). GTX638125")