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ADFP antibody [N1C2]

Anti-ADFP antibody [N1C2] used in Western Blot (WB). GTX110204

Cat. No. GTX110204

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Sheep, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADFP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perilipin 2 , ADFP , ADRP

Cellular Localization

Membrane

Background

Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ADFP antibody [N1C2] used in Western Blot (WB). GTX110204

GTX110204 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [N1C2] (GTX110204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ADFP antibody [N1C2] Cat. No. GTX110204

Rating ( Average 3 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 01-Oct-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Huh7 cells
Blocking : 4% BSA, 25°C, 0.5Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.2% triton
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 26-Sep-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat skin milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169