APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human PLIN2 (NP_001113.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
perilipin 2 , ADFP , ADRP
Cellular Localization
Membrane
Background
The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX54569 WB Image
WB analysis of various sample lysates using GTX54569 ADFP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54569 IHC-P Image
IHC-P analysis of rat liver tissue using GTX54569 ADFP antibody.
Dilution : 1:50
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GTX54569 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX54569 ADFP antibody.
Blue : DAPI
Dilution : 1:100
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GTX54569 WB Image
WB analysis of rat heart tissue lysate using GTX54569 ADFP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54569 WB Image
WB analysis of HepG2 cell lysate using GTX54569 ADFP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54569 IHC-P Image
IHC-P analysis of human liver tissue using GTX54569 ADFP antibody.
Dilution : 1:50
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GTX54569 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX54569 ADFP antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
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REVIEW
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