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ADHFE1 antibody [N1C1]

Anti-ADHFE1 antibody [N1C1] used in Western Blot (WB). GTX119264
Anti-ADHFE1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX119264
Anti-ADHFE1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119264

Cat. No. GTX119264

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ADHFE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADHFE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722811

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alcohol dehydrogenase iron containing 1 , ADH8 , HMFT2263 , HOT

Cellular Localization

Mitochondrion

Background

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ADHFE1 antibody [N1C1] used in Western Blot (WB). GTX119264

GTX119264 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX119264 diluted at 1:1000

Anti-ADHFE1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX119264

GTX119264 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ADHFE1(GTX119264) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ADHFE1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119264

GTX119264 ICC/IF Image

ADHFE1 antibody [N1C1] detects ADHFE1 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: ADHFE1 protein stained by ADHFE1 antibody [N1C1] (GTX119264) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169