APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Rat lung , HepG2 , mouse lung
Predict Reactivity
Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ADI1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617999
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acireductone dioxygenase 1 , APL1 , ARD , Fe-ARD , HMFT1638 , MTCBP1 , Ni-ARD , SIPL , mtnD
Cellular Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side , Nucleus
Background
This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. A pseudogene that is located on chromosome 20 has been defined for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX118578 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ADI1(GTX118578) antibody at 1:200 dilution.
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GTX118578 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX118578 diluted at 1:2000
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GTX118578 IHC-P Image
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using ADI1(GTX118578) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118578 WB Image
ADI1 antibody detects ADI1 protein by Western blot analysis.
A. 50 μg mouse lung lysate/extract
12 % SDS-PAGE
ADI1 antibody (GTX118578) dilution: 1:1000
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GTX118578 WB Image
ADI1 antibody detects ADI1 protein by Western blot analysis.
A. 50 μg Rat lung lysate/extract
12 % SDS-PAGE
ADI1 antibody (GTX118578) dilution: 1:1000
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REFERENCE
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REVIEW
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