GeneTex
United States (US)

ADPGK antibody [N2C3]

GTX106029_WB_19011506_908.jpg
GTX106029_39932_IHC_19011506_362.jpg

Cat No. GTX106029

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADPGK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

2610017G09Rik antibody, ADP-GK antibody, DKFZp434B195 antibody, ADPGK antibody, ATP-dependent glucokinase antibody, rbBP-35 antibody, ADP-dependent glucokinase antibody

Cellular Localization

Secreted

Background

ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX106029_WB_19011506_908.jpg

GTX106029 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX106029 diluted at 1:500

GTX106029_39932_IHC_19011506_362.jpg

GTX106029 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ADPGK(GTX106029) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169