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ADPGK antibody [N2C3]

Anti-ADPGK antibody [N2C3] used in Western Blot (WB). GTX106029
Anti-ADPGK antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106029

Cat. No. GTX106029

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ADPGK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HeLaS3 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ADPGK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP dependent glucokinase , 2610017G09Rik , ADP-GK

Cellular Localization

Secreted

Background

ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ADPGK antibody [N2C3] used in Western Blot (WB). GTX106029

GTX106029 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX106029 diluted at 1:500

Anti-ADPGK antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX106029

GTX106029 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ADPGK(GTX106029) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169