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ADSL antibody [AT16C10]

Anti-ADSL antibody [AT16C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57653
Anti-ADSL antibody [AT16C10] used in Western Blot (WB). GTX57653
Anti-ADSL antibody [AT16C10] used in Flow cytometry (FACS). GTX57653

Cat. No. GTX57653

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT16C10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all ADSL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT16C10 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ADSL protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylosuccinate lyase , ADSL , AMPS , ASASE , ASL

Background

The protein encoded by this gene belongs to the lyase 1 family. It is an essential enzyme involved in purine metabolism, and catalyzes two non-sequential reactions in the de novo purine biosynthetic pathway: the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR) and the conversion of adenylosuccinate (S-AMP) to adenosine monophosphate (AMP). Mutations in this gene are associated with adenylosuccinase deficiency (ADSLD), a disorder marked with psychomotor retardation, epilepsy or autistic features. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-ADSL antibody [AT16C10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57653

GTX57653 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57653 ADSL antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-ADSL antibody [AT16C10] used in Western Blot (WB). GTX57653

GTX57653 WB Image

WB analysis of various samples using GTX57653 ADSL antibody.
Lane 1 : Recombinant Human ADSL protein (20 ng)
Lane 2 : HeLa whole cell lysate (40 μg)
Lane 3 : 293T whole cell lysate (40 μg)
Lane 4 : Jurkat whole cell lysate (40 μg)
Lane 5 : HepG2 whole cell lysate (40 μg)
Lane 6 : A549 whole cell lysate (40 μg)
Lane 7 : MCF-7 whole cell lysate (40 μg)

Anti-ADSL antibody [AT16C10] used in Flow cytometry (FACS). GTX57653

GTX57653 FACS Image

FACS analysis of Hep3B cells using GTX57653 ADSL antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399