GeneTex
  • Country / Location Selection

United States (US)

AEC

Cat No. GTX30938

Application IHC
Package
1 kit ($149)
PRODUCT

Product Description

AEC
APPLICATION

Application Note

To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. Add one drop of reagent C chromogen, mix well. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. Add few drops of ready to use AEC reagent, incubate at RT or 37ºC for 5-10 minutes. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. Add counterstain compatible with AEC (we recommend hematoxylin counterstain for 30-60 seconds). Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
PROPERTIES

Form

Liquid

Storage

2-8ºC

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin nuclear counterstain. This chromogen is soluble in alcohol and organic solvents therefore cannot be mounted with organic mounting medium. We recommend our aqueous mounting medium ImmunoHistoMount (IHM) for mounting of this chromogen.
REFERENCE
REVIEW
There are currently no reviews for AEC (GTX30938). Be the first to share your experience with this product.
Package List Price ($)
$ 149