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United States (US)

AEC

Cat. No. GTX30938

Reference 1

Application

 IHC
Package
1 kit ($159)
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PRODUCT

Summary

AEC

APPLICATION

Application Note

To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. Add one drop of reagent C chromogen, mix well. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. Add few drops of ready to use AEC reagent, incubate at RT or 37ºC for 5-10 minutes. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. Add counterstain compatible with AEC (we recommend hematoxylin counterstain for 30-60 seconds). Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.

PROPERTIES

Form

Liquid

Storage

Store at 2-8ºC.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin nuclear counterstain. This chromogen is soluble in alcohol and organic solvents therefore cannot be mounted with organic mounting medium. We recommend our aqueous mounting medium ImmunoHistoMount (IHM) for mounting of this chromogen.

REFERENCE

Application Reference

Yu F et al. Mol Cancer Res 2013; 11 (8) : 923-36 miR-155-deficient bone marrow promotes tumor metastasis.
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Package List Price ($)
$ 159