APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 4 μg/mL |
10 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Cell Lysate , HeLa Cell Slide(GTX25529) , HEK293 Cell Lysate(GTX27902)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES.The immunogen is located within the last 50 amino acids of AES.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TLE family member 5, transcriptional modulator , AES , AES-1 , AES-2 , ESP1 , GRG , GRG5 , Grg-5
Cellular Localization
Nucleus
Background
The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX16982 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX16982 AES antibody. Working concentration : 10 μg/ml
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GTX16982 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX16982 AES antibody. Working concentration : 20 μg/ml
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GTX16982 WB Image
WB analysis of 293 cell lysate using GTX16982 AES antibody. Working concentration : (A) 1, (B) 2, and (C) 4 μg/ml
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REFERENCE
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REVIEW
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