GeneTex
United States (US)

AF10 antibody [C3], C-term

GTX106284_39778_IHC_19011506_226.jpg
GTX106284_WB_19011506_889.jpg

Cat No. GTX106284

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

7.29mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AF10. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AF10 antibody, DKFZp686E10210 antibody, MGC75086 antibody, MLLT10 antibody, type III AF10 protein antibody, ALL1-fused gene from chromosome 10 protein antibody, type IV AF10 protein antibody, protein AF-10 antibody, type I AF10 protein antibody, "myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 10 antibody"

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX106284_39778_IHC_19011506_226.jpg

GTX106284 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC xenograft, using AF10(GTX106284) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106284_WB_19011506_889.jpg

GTX106284 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
AF10 antibody
GTX106284 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169