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AF9 antibody

Anti-AF9 antibody used in Western Blot (WB). GTX102835
Anti-AF9 antibody used in Western Blot (WB). GTX102835
Anti-AF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX102835
Anti-AF9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102835

Cat No. GTX102835

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ChIP assay, ChIP-seq

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ChIP assay Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , THP-1 , MLLT3 shRNA-transfected 293T , *SEM-K2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MLLT3 super elongation complex subunit , AF9 , YEATS3

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-AF9 antibody used in Western Blot (WB). GTX102835

GTX102835 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AF9 antibody used in Western Blot (WB). GTX102835

GTX102835 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AF9 antibody used in IHC (Paraffin sections) (IHC-P). GTX102835

GTX102835 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9(GTX102835) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AF9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102835

GTX102835 ICC/IF Image

AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AF9 protein stained by AF9 antibody (GTX102835) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

REFERENCE

REVIEW

AF9 antibody Cat No. GTX102835

Rating ( Average 4 based on 1 users reviews)
Application
ChIP assay(ChIP assay) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2013
Score :
Application Tested : ChIP assay
Sample Species : Hu
Sample : SEM-K2 cells
Amount used : 1μg
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : ChIP was performed with SEM-K2 chromatin extract and 1 ug of either normal rabbit IgG (red bar) or anti-AF9 antibody (GTX102835; blue bar). The precipitated DNA was detected by real-time PCR with primer set targeting to HOXA9, MEIS1 or CTNNB1. The ChIP result is shown as fold enrichment relative to a negative control region within NANOG locus.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169