GeneTex
United States (US)

AF9 antibody

KO/KD
GTX102835_42732_20181221_WB_shRNA_watermark_19011519_720.jpg
GTX102835_40835_IHC_19011506_139.jpg
GTX102835_42732_20170419_IFA_19011506_630.jpg
GTX102835_42732_20170126_WB_19011506_394.jpg

Cat No. GTX102835

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , THP-1 , MLLT3 shRNA-transfected 293T , *SEM-K2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AF9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mllt3, Super Elongation Complex Subunit,Af9,Yeats3,Mllt3

Cellular Localization

Nucleus

Database

DATA IMAGES
GTX102835_42732_20181221_WB_shRNA_watermark_19011519_720.jpg

GTX102835 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX102835_40835_IHC_19011506_139.jpg

GTX102835 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9(GTX102835) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102835_42732_20170419_IFA_19011506_630.jpg

GTX102835 ICC/IF Image

AF9 antibody detects AF9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AF9 protein stained by AF9 antibody (GTX102835) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

GTX102835_42732_20170126_WB_19011506_394.jpg

GTX102835 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 antibody (GTX102835) diluted at 1:1000.

REVIEW

AF9 antibody Cat No. GTX102835

Rating ( Average 4 based on 1 users reviews)
Application
ChIP assay(ChIP assay) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2013
Score :
Application Tested : ChIP assay
Sample Species : Hu
Sample : SEM-K2 cells
Amount used : 1μg
Primary Antibody : 1:100, 4°C, overnightHr
Figure Note : ChIP was performed with SEM-K2 chromatin extract and 1 ug of either normal rabbit IgG (red bar) or anti-AF9 antibody (GTX102835; blue bar). The precipitated DNA was detected by real-time PCR with primer set targeting to HOXA9, MEIS1 or CTNNB1. The ChIP result is shown as fold enrichment relative to a negative control region within NANOG locus.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169