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AFAP antibody

Anti-AFAP antibody used in Western Blot (WB). GTX31462

Cat. No. GTX31462

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa Cell Lysate

Product Note

Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1.The immunogen is located within amino acids 30 - 80 of AFAP1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin filament associated protein 1 , AFAP , AFAP-110 , AFAP110

Background

The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES

Anti-AFAP antibody used in Western Blot (WB). GTX31462

GTX31462 WB Image

WB analysis of HeLa cell lysate using GTX31462 AFAP antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399