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AFG3L2 antibody [N1N2], N-term

Orthogonal
Anti-AFG3L2 antibody [N1N2], N-term used in Western Blot (WB). GTX102036
Anti-AFG3L2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102036

Cat. No. GTX102036

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all AFG3L2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

SK-N-SH membrane fraction extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AFG3L2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171320

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AFG3 like matrix AAA peptidase subunit 2 , SCA28 , SPAX5

Cellular Localization

Mitochondrion membrane

Background

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AFG3L2 antibody [N1N2], N-term used in Western Blot (WB). GTX102036

GTX102036 WB Image

Orthogonal

AFG3L2 antibody detects AFG3L2 protein by western blot analysis. SK-N-SH whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AFG3L2 antibody (GTX102036) diluted at 1:1000.

Anti-AFG3L2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102036

GTX102036 IHC-P Image

AFG3L2 antibody [N1N2], N-term detects AFG3L2 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
AFG3L2 antibody [N1N2], N-term (GTX102036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

S Akabane et al. Cell Rep 2023; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria
Application : WB
Reactivity : Human
Correia SP et al. Sci Rep 2019; 9 (1) : 198 The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169