GeneTex
United States (US)

AFG3L2 antibody [N1N2], N-term

Orthogonal
GTX102036_40807_20150820_WB_Fraction_19011506_208.jpg
GTX102036_40807_20151221_IHC-P_R_19011506_832.jpg

Cat No. GTX102036

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

SK-N-SH membrane fraction extract

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human AFG3L2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Afg3 Like Matrix Aaa Peptidase Subunit 2,Sca28,Spax5,Afg3L2

Cellular Localization

Mitochondrion membrane

Background

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102036_40807_20150820_WB_Fraction_19011506_208.jpg

GTX102036 WB Image

Orthogonal

AFG3L2 antibody detects AFG3L2 protein by western blot analysis. SK-N-SH whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AFG3L2 antibody (GTX102036) diluted at 1:1000.

GTX102036_40807_20151221_IHC-P_R_19011506_832.jpg

GTX102036 IHC-P Image

AFG3L2 antibody [N1N2], N-term detects AFG3L2 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
AFG3L2 antibody [N1N2], N-term (GTX102036) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169