Human plasma was separated by 7.5% SDS-PAGE, and the membrane was blotted with AFM antibody (GTX112390) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AFM antibody detects AFM protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
AFM antibody (GTX112390) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min