GeneTex
United States (US)

AGAP1 antibody [C3], C-term

GTX106298_WB_19011506_706.jpg
GTX106298_39701_20160728_WB_R_19011506_252.jpg
GTX106298_IFA_19011506_184.jpg
GTX106298_39701_20160728_WB_M_19011506_310.jpg

Cat No. GTX106298

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

HepG2 , C8D30 , NIH-3T3 , C2C12 , HeLa , Rat2

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CENTG2 antibody, GGAP1 antibody, KIAA1099 antibody, MGC71657 antibody, AGAP1 antibody, centaurin-gamma-2 antibody, "centaurin, gamma 2 antibody", GTP-binding and GTPase-activating protein 1 antibody, "Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 1 antibody", "arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 antibody", "ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 antibody"

Cellular Localization

Cytoplasm

Background

CENTG2 belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin cytoskeleton dynamics (Nie et al., 2002 [PubMed 12388557]).[supplied by OMIM]
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Database

DATA IMAGES
GTX106298_WB_19011506_706.jpg

GTX106298 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106298 diluted at 1:1000

GTX106298_39701_20160728_WB_R_19011506_252.jpg

GTX106298 WB Image

Whole cell extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AGAP1 antibody [C3], C-term (GTX106298) diluted at 1:1000.

GTX106298_IFA_19011506_184.jpg

GTX106298 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CENTG2(GTX106298) antibody at 1:200 dilution.

GTX106298_39701_20160728_WB_M_19011506_310.jpg

GTX106298 WB Image

Various whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGAP1 antibody [C3], C-term (GTX106298) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169