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AGAP1 antibody [C3], C-term

Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298
Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298
Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298
Anti-AGAP1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106298

Cat. No. GTX106298

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all AGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

HepG2 , C8D30 , NIH-3T3 , C2C12 , Rat2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240620

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 , AGAP-1 , CENTG2 , GGAP1 , cnt-g2

Cellular Localization

Cytoplasm

Background

CENTG2 belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin cytoskeleton dynamics (Nie et al., 2002 [PubMed 12388557]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298

GTX106298 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX106298 diluted at 1:1000
Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298

GTX106298 WB Image

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AGAP1 antibody [C3], C-term (GTX106298) diluted at 1:1000.

Anti-AGAP1 antibody [C3], C-term used in Western Blot (WB). GTX106298

GTX106298 WB Image

Whole cell extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AGAP1 antibody [C3], C-term (GTX106298) diluted at 1:1000.

Anti-AGAP1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106298

GTX106298 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CENTG2(GTX106298) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169