APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 2.5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89393-PEP).
Product Note
This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
argonaute RISC component 1 , EIF2C , EIF2C1 , GERP95 , Q99 , hAgo1
Background
This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
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GTX89393 IHC-P Image
IHC-P analysis of human small intestine using GTX89393 AGO1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89393 WB Image
WB analysis of HEK293 overexpressing AGO1 (non-transfected HEK293 in lane B) using GTX89393 AGO1 antibody, Internal.
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GTX89393 IHC-P Image
IHC-P analysis of human placenta using GTX89393 AGO1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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REFERENCE
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REVIEW
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