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AGR3 antibody

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582
Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582
Anti-AGR3 antibody used in Western Blot (WB). GTX100582
Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582
Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582
Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

Cat No. GTX100582

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

A549 , MCF-7

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AGR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

anterior gradient 3, protein disulphide isomerase family member , AG-3 , AG3 , BCMP11 , HAG3 , PDIA18 , hAG-3

Cellular Localization

Secreted

Database

DATA IMAGES

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

GTX100582 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using AGR3(GTX100582) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

GTX100582 IHC-P Image

AGR3 antibody detects AGR3 protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
AGR3 antibody (GTX100582) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AGR3 antibody used in Western Blot (WB). GTX100582

GTX100582 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: MCF-7
12% SDS PAGE
GTX100582 diluted at 1:1000

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

GTX100582 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using AGR3(GTX100582) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

GTX100582 IHC-P Image

AGR3 antibody detects AGR3 protein at cytoplasm in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
AGR3 antibody (GTX100582) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AGR3 antibody used in IHC (Paraffin sections) (IHC-P). GTX100582

GTX100582 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using AGR3(GTX100582) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169