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AGRP antibody, C-term

Anti-AGRP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89559

Cat. No. GTX89559

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Fr

Reactivity

 Human
Package
100 μg ($399)
See all AGRP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr 0.05-0.1μg/ml

Note :

IHC-Fr
Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons.
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89559-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1; NP_015531.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

agouti related neuropeptide , AGRT , ART , ASIP2

Cellular Localization

Secreted

Background

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

Database

DATA IMAGES

Anti-AGRP antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89559

GTX89559 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamus using GTX89559 AGRP antibody, C-term.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.05μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399