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AGTR1 antibody [HL2524]

Recombinant antibody Orthogonal
Anti-AGTR1 antibody [HL2524] used in IHC (Paraffin sections) (IHC-P). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

Cat. No. GTX638885

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2524

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AGTR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human AGTR1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin II receptor type 1 , AG2S , AGTR1B , AT1 , AT1AR , AT1B , AT1BR , AT1R , AT2R1 , HAT1R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-AGTR1 antibody [HL2524] used in IHC (Paraffin sections) (IHC-P). GTX638885

GTX638885 IHC-P Image

AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded Huh-7 xenograft.
AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Orthogonal

Huh-7 cytoplasma extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (CE: cytoplasma extract; ME: membrane extract)

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-AGTR1 antibody [HL2524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638885

GTX638885 ICC/IF Image

AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane by immunofluorescent analysis.
Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations