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AGTR1 antibody, Internal

Anti-AGTR1 antibody, Internal used in Western Blot (WB). GTX89149

Cat. No. GTX89149

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all AGTR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89149-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EIQKNKPRNDDIFK, from the internal region of the protein sequence according to NP_000676.1; NP_004826.2; NP_033611.1; NP_114038.1; NP_114438.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

angiotensin II receptor type 1 , AG2S , AGTR1B , AT1 , AT1AR , AT1B , AT1BR , AT1R , AT2R1 , HAT1R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-AGTR1 antibody, Internal used in Western Blot (WB). GTX89149

GTX89149 WB Image

WB analysis of mouse liver lysate using GTX89149 AGTR1 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Nemoto T et al. PLoS One 2020; 15 (8) : e0238223 Elevated blood pressure in high-fat diet-exposed low birthweight rat offspring is most likely caused by elevated glucocorticoid levels?due to abnormal pituitary negative feedback.
Sun Y et al. Cell Death Dis 2020; 11 (6) : 432 Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399