APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:25 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium Azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.
Purification
Purified by protein A column followed by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AGT,AGT1,AGXT,AGXT1,PH1,SPAT,SPT,TLH6,alanineglyoxylate aminotransferase,alanine--glyoxylate and serine--pyruvate aminotransferase
Cellular Localization
Peroxisome,Mitochondrion matrix
Background
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04936 WB Image
WB analysis of human liver tissue lysate using GTX04936 AGXT antibody.
Loading : 20μg
Dilution : 1:8000
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GTX04936 IHC-P Image
IHC-P analysis of human hepatic carcinoma tissue using GTX04936 AGXT antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25
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GTX04936 IHC-P Image
IHC-P analysis of human hepatocarcinoma tissue using GTX04936 AGXT antibody.
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GTX04936 WB Image
WB analysis of HepG2 whole cell lysate using GTX04936 AGXT antibody.
Loading : 35 μg
Dilution : 8μg/ml
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GTX04936 FACS Image
FACS analysis of HepG2 cells using GTX04936 AGXT antibody.
Green line : Primary antibody
Blue line : Neative control
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GTX04936 WB Image
WB analysis of human liver tissue lysate using GTX04936 AGXT antibody.
Loading : 20 μg
Dilution : 1:2000
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REFERENCE
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REVIEW
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